Andreia Pinto , PhD

Advanced Workflow Specialists Manager, Leica Microsystems

Andreia Pinto started her career as a Biomedical Scientist in Histocellular Pathology with a particular interest in electron microscopy. In 2014, she worked at the Primary Ciliary Diagnosis (PCD) in Lisbon. In 2019, Andreia moved to the Royal Brompton Hospital, London, working as a Thoracic Research Associate, where she was responsible for training a deep machine learning platform to recognize patterns in EM images of cilia in diagnosing PCD. She completed her PhD on this topic in 2022 while also investigating new insights into SARS-CoV-2 infection of the respiratory airway.Andreia is currently an Advanced Workflow Application Specialists Manager for EM Sample Preparation with Leica Microsystems.

Waffle method lamella preparation and cryo-ET results - Representative workflow and results of lamella preparation from a vitrified C. elegans L1 larva using the waffle method. Image courtesy: Prof. Dr. Jürgen M. Plitzko - doi.org/10.1038/s41592-023-02113-5-

Waffle Method Workflow: From HPF to Cryo-ET Lamellae

Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella…

Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

从显微镜到电镜：完整的冷冻光电联用工作流程

在题为“多模态玻璃化征程，从实验台到电子显微镜的冷冻关联工作流程”的网络研讨会上，专家团队（Edoardo D'Imprima、Zhengyi Yang、Andreia Pinto 和 Martin…