Thomas Veitinger , Dr.

Thomas Veitinger

Dr. Thomas Veitinger studied Biology at the Ruhr-University Bochum, Germany and received a diploma in Developmental Neurobiology. In his Ph.D. thesis at the Department of Cell Physiology of the Ruhr-University in Bochum, he investigated odor-driven subcellular calcium dynamics of human spermatozoa and gained experience in live-cell imaging methods and general physiology. As a post-doc, he worked in the department for chemosensation of the RWTH Aachen University, Germany lead by Prof. Dr. Marc Spehr. There, he investigated male germ stem cells using the patch-clamp technique and live-cell imaging. Since May 2011, he is employed by Leica Microsystems as an Application Manager for live-cell imaging, electrophysiology and neuroscience applications.

Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.

比例成像

细胞的许多基本功能在很大程度上依赖于离子(例如钙、镁)、电压势和细胞质与周围细胞外空间之间的 pH 值的微妙但动态的平衡。这些平衡的变化会显著改变细胞的行为和功能。因此,实时测量细胞内离子、电压和 pH…

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
Primary leaves of cowpea (Vigna unguiculata "California Blackeye") inoculated with cowpea mosaic virus (CPMV) containing the GFP-gene inserted between the movement protein (MP) and the capsid proteins (CPs) in the viral RNA 2

活细胞成像简介

了解复杂且快速变化的细胞动力学是深入探索生物进程的重要一步。因此,现代生命科学研究越来越需要关注于在分子水平上实时发生的生理事件。
微管蛋白的 TIRF 图像,YFP 标记,穿透深度:120 毫米

全内反射荧光显微镜(total internal reflection fluorescent microscope,TIRFM)在生命科学研究中的应用

全内反射荧光显微镜的独特之处在于利用衰逝波激发荧光团。与传统的弧光灯、LED 或激光宽场荧光照明方式不同,衰逝波仅能从盖玻片/介质界面开始穿透样本约 100 纳米深度。
Modulation contrast visualizes transparent, low-contrast specimens.

集成调制对比度 (IMC)

霍夫曼调制对比已成为观察未染色、低对比生物标本的标准。其创新的技术实现大大简化了操作,提高了使用的灵活性。将调制器集成到现代倒置显微镜的光束路径中,就可以使用各种明视野或相位物镜,而无需选择少量专用物镜。
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