Constantin Kappel , Dr.
Dr. Constantin Kappel originally studied Biochemistry at the University of Tübingen in Germany and at the University of Toronto in Canada. In 2003 he moved to the German Cancer Research Centre (DKFZ) in Heidelberg to study with Prof Dr. Roland Eils in the Department of Theoretical Bioinformatics and in 2007 he joined Leica Mikrosysteme Vertrieb GmbH as European Product Specialist FCS/FLIM. He obtained his Doctoral degree from the University of Heidelberg in 2009. From 2010 until 2025 he worked as Product Manager, Confocal Microscopy and Senior AI Engineer, Image Intelligence for Leica Microsystems CMS GmbH, Mannheim, Germany.
荧光寿命成像与荧光共振能量转移
荧光寿命是荧光团在发射荧光光子返回基态之前保持其激发态的平均时间长度。这取决于荧光团的分子组成和纳米环境。
FLIM将寿命测量与成像相结合：对每个图像像素以测得的荧光寿命进行颜色编码，产生额外的图像反差。因此，FLIM可以提供关于荧光分子空间分布的信息和有关其生化状态或纳米环境的信息。…
荧光相关光谱（FCS）
荧光相关光谱学（FCS）通过测量亚飞升体积内荧光强度的波动来检测扩散时间、分子数量或荧光标记分子的暗态等参数。这项技术是在20世纪70年代初期由瓦特·韦伯（Watt Webb）和鲁道夫·里格勒（Rudolf Rigler）独立开发的。
