Cveta Tomova , Dr.

Cveta Tomova

Dr. Cveta Tomova was born in Bulgaria where she completed her MSc degree in Biology, specializing in medical and veterinary parasitology, a field in which she later worked as research associate in the Institute of Veterinary Medicine.

In 2004 she moved to Germany where she was a research associate in Institute of Zoology, TU-Dresden. During this time she began her PhD which was completed at the University of Utrecht (The Netherlands), with Prof. Arie Verkleij. She gained experience in EM sample preparation techniques and 3D-EM Tomography. Before joining Leica Microsystems, Cveta was a postdoctoral fellow in the Dutch Cancer Institute, working with Prof. Peter Peters on techniques such as CEMOVIS.

Dr. Cveta Tomova was Product Manager at  Leica Microsystems in Vienna, Austria.

Electron microscope (EM) image of a cross section of C. elegans (roundworm). Courtesy of T. Müller-Reichert, MPI-CBG, Dresden, Germany and K. McDonald, University of California, Berkeley, USA.

高压冷冻简介

水是细胞最主要的组成部分,因此对于维持细胞超微结构至关重要。目前,冷冻固定是固定细胞成分,而不导致其显著结构变化的唯一途径。现阶段有两种常见的方法:投入冷冻与高压冷冻固定。

视频教程:高压冷冻不同载具的填充与组装

高压冷冻(HPF)是一种低温固定方法,主要用于生物样本,也可用于各种非生物材料。这种技术是对多种细胞类型和组织,或有机和无机复合材料的最佳保存方法。通常情况下,高压冷冻样本在经过适当处理后,会用光学或电子显微镜进行进一步分析。

关于高压冷冻仪中液氮的热力学考虑

在高压冷冻过程中,待冷冻样品和冷却剂的相变导致我们对使用液氮作为冷冻剂提出一定的质疑。根据氮气的相图(图1),温度或压力的变化将改变该元素的状态。在环境压力下,氮气在-209.86℃至-195.80℃之间为液态。在有限的相位边界内,它是一个相当脆弱的平衡。
Scroll to top