Leica EM UC7 超薄切片机和冷冻超薄切片机 电镜样品制备 产品 首页 Leica Leica Microsystems

For Perfect Sectioning at Room Temperature and Cryo

为室温理想切片设计的超薄切片机和为冷冻无缺陷切片的冷冻超薄切片机 Leica EM UC7

  • 材料科学中的样品制备

    样品制备是以高分辨率观察样品表面之上的精细纹理和结构的关键。得心应手的预制备工作流程和工具将带来制备出色的样品,缩短流程时间,并产生精确可靠的结果。
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    细胞培养

    在实验室环境下培养细胞是科学家在细胞生物学、癌症研究、发育生物学等任何类型生命科学及药物研究领域开展工作的基础。了解徕卡如何帮助您在实验室中培养动物细胞。
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    斑马鱼研究

    为了在筛选、分拣、操作和成像过程中获取高质量结果,您需要观察细节和结构,从而为您的下一步研究做出正确的决策。 徕卡体视显微镜和透射光底座以出众的光学器件和优良的分辨率而闻名,是全世界研究学者的首选。
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    荧光

    荧光是生物和分析显微镜中最常用的物理现象之一,主要是因为它具有灵敏度高、特异性强的特点。荧光是冷发光的一种形式。
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  • 地质、环境、古生物和地球科学显微镜

    利用徕卡显微系统提供的多功能、可靠的显微镜系统,研究和发现常规至具有挑战性的环境保护和地球科学问题,提出解决方案。您的工作对于推进我们对周围世界的理解举足轻重,因此必须使用功能强大而且准确的研究工具,良好地记录并再现结果。徕卡的生产线符合人体工学,高品质的显微镜解决方案所提供的模块化系统,有助于精确测量、细致分析各种材料。
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  • 生命科学

    作为生物学教师,您使用组织切片和血液涂片作为显微镜样本,向您的学生展示生物体的结构。
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  • Science Lab Topic: EM Sample Preparation

    Exploring the Structure and Life Cycle of Viruses

    The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
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  • Science Lab Topic: EM Sample Preparation

    Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy

    A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
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  • Science Lab Topic: EM Sample Preparation

    Workflows and Instrumentation for Cryo-electron Microscopy

    Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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  • Science Lab Topic: Widefield Microscopy

    用于病毒复制研究的正置荧光显微镜

    冠状病毒2致重度急性呼吸综合征(SARS-CoV-2)出现于2019年末,并快速传播全世界。由于其大面积的影响,研究人员对病毒的性质进行了深入的研究以期最终阻止大流行。一个重要的方面是病毒如何在宿主细胞中复制。Ogando及其同事的研究已经揭示了SARS-CoV-2的复制动力学、适应能力和细胞病理学。他们的工具之一是用荧光显微镜观察SARS-CoV-2感染的VeroE6细胞。通过使用前一段时间产生…
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  • Science Lab Topic: Confocal Microscopy

    显微镜在病毒学中的应用

    引起新型冠状病毒肺炎(Covid-19)的冠状病毒SARS-CoV-2肆虐全球并影响了我们生活的方方面面。对于免疫和治疗方法的搜索研究(即如何抗击该病毒)成为了2020年全人类的第一要务。显微镜在这类研究中起着重要作用。为了了解受体结合、基因组释放、复制、装配和病毒出芽的基本原理以及我们的免疫系统效应,可以使用不同的方法和显微镜。本文概述了为什么显微镜是病毒学和感染生物学的重要工具,并举例说明了不…
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  • Science Lab Topic: EM Sample Preparation

    Workflow Solutions for Industrial Research

    This brochure presents and explains appropriate workflow solutions for the most frequently required sample preparation methods for material science samples.
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  • Science Lab Topic: Laser Microdissection

    Medicinal Use of Qianhu Plant Roots

    This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn (Qianhu in Chinese). The qualitative and quantitative…
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  • Science Lab Topic: EM Sample Preparation

    Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells

    Intracellular dynamic events, such as protein trafficking, recycling, and degradation as well as signal transduction, to name a few, can be studied using live-cell imaging techniques. However,…
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  • Science Lab Topic: EM Sample Preparation

    超薄切片介绍

    对样本开展研究时,为了以纳米级分辨率显示其精细结构,通常会使用到电子显微镜。电子显微镜有两种类型:扫描电子显微镜(SEM)用于对样本表面成像,以及需要使用极薄电子透明样本的透射电子显微镜(TEM)。因此,使用电子显微镜对样本内部的精细结构进行成像时,此类技术解决方案需要制作出非常薄的样本切片。被称为超显微技术的样本制备方法可以产生具有最小伪影的超薄切片(厚度20-150nm)。在切片过程中,样本的…
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  • Science Lab Topic: EM Sample Preparation

    Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation

    Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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  • Science Lab Topic: EM Sample Preparation

    Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue

    Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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  • Science Lab Topic: EM Sample Preparation

    Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions

    Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being…
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  • Science Lab Topic: EM Sample Preparation

    Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast

    Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…
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  • Science Lab Topic: EM Sample Preparation

    Immuno - Electron Microscopy of High Pressure Frozen and Freeze Substituted Mouse Heart

    Application Note for Leica EM AFS2 - Mouse heart tissue from wild-type (WT) and αT -catenin KO (KO) mice was excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer Leica…
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  • Science Lab Topic: EM Sample Preparation

    Cell Envelope Structure of a Gram-negative Thermotolerant y-Proteobacterium Acidithiobacillus sp., Strain HV2/2 and its Interaction with Pyrite

    Application Note for Leica EM AFS2 - Cells of the the moderately thermophilic Acidithiobacillus sp.strain HV2/2, were centrifuged at 20,0009xg and then cryo-immobilized by high-pressure freezing on…
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  • Science Lab Topic: EM Sample Preparation

    Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite

    Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…
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  • Science Lab Topic: EM Sample Preparation

    Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells

    Application Note for Leica EM AFS2 - Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer. Freeze…
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  • Science Lab Topic: EM Sample Preparation

    Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”

    In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…
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  • Science Lab Topic: EM Sample Preparation

    Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa

    Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions.…
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  • Science Lab Topic: CLEM

    Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

    We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…
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  • Science Lab Topic: Confocal Microscopy

    Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells

    The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale…
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  • Science Lab Topic: EM Sample Preparation

    Protein Trafficking in Cereal Seeds

    Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to…
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  • Science Lab Topic: EM Sample Preparation

    Perusing Alternatives for Automated Staining of TEM Thin Sections

    Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…
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  • Science Lab Topic: EM Sample Preparation

    Atomic Force Microscopy Study of a Stretched Impact Copolymer

    Atomic force microscopy (AFM) is a powerful tool in the suite of nanoscale characterization techniques that provides a variety of information including topography, mechanical properties, and…
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  • Science Lab Topic: EM Sample Preparation

    Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

    This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with…
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  • Science Lab Topic: CLEM

    Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections

    Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic…
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