暗场显微镜

A darkfield microscope is similar to a conventional brightfield microscope, except it uses an annular aperture in front of the light source. The light from darkfield illumination impinges onto the specimen or sample at a high angle of incidence, either transmits through the sample or reflects off its surface, then passes through the objective lens, and finally goes through the eyepieces or reaches the camera sensor. Darkfield illumination causes uniform areas of transparent samples or flat surfaces of opaque samples to appear dark, as the vast majority of the light at the high incident angle misses the objective. Normally there are features in transparent samples or on the surface of opaque samples which scatter light. For this reason, darkfield microscopy images show a dark background with brighter areas corresponding to these features, because the light they scatter enters into the objective.

Yes, a live biological specimen, such as cells or an organism, can be observed easily with a darkfield microscope.

Yes, a darkfield microscope can be equipped with a camera for recording images observed with the contrast method. It can also be equipped with other accessories. For more information, contact your local Leica representative.