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STELLARIS 5 & STELLARIS 8 共聚焦显微镜平台

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Spheroid stained with Cyan: Dapi nuclear countertain; Green AF488 Involucrin; Orange AF55 Phalloidin Actin; Magenta AF647 CK14.

Notable AI-based Solutions for Phenotypic Drug Screening

Learn about notable optical microscope solutions for phenotypic drug screening using 3D-cell culture, both planning and execution, from this free, on-demand webinar.
[Translate to chinese:] Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with the THUNDER Imager 3D Cell Culture. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

如何深入了解类器官和细胞球模型

在本电子书中,您将了解3D细胞培养模型(如类器官和细胞球)成像的关键注意事项。探索创新型显微镜解决方案,来实时记录类器官和细胞球的动态成像过程。
Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.

Epi-Illumination Fluorescence and Reflection-Contrast Microscopy

This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Application example of hyperspectral imaging

多重共焦成像在癌症研究和免疫学中的潜力

在本次网络研讨会上,来自莫纳什制药科学研究所的 Cameron Nowell 和他的同事将分享他们在多重成像方面的经验,以及他们通过巧妙的共聚焦成像采集和利用 FLIM 等其他多重成像模式所取得的成果。
[Translate to chinese:] Molecular structure of the green fluorescent protein (GFP)

荧光蛋白简介

本文概述了荧光蛋白及其光谱特性。随着 20 世纪 50 年代末荧光蛋白的发现,荧光显微技术发生了巨大变化。它始于 O. Shimomura 和来自水母(Aequorea victoria)的绿色荧光蛋白(GFP)[1]。后来出现了数百种 GFP…
Multi-tissue array with 4 markers shown including DAPI, NaKATPase, PanCk, and Vimentin.

Spatial Biology: Learning the Landscape

Spatial Biology: Understanding the organization and interaction of molecules, cells, and tissues in their native spatial context
[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

神经血管病理学之窗

探索先天性免疫如何在神经血管病变后持续产生有害影响,以及能够对这些事件进行纵向研究的技术发展。
[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

可重复性、协作和新成像技术的力量

在本次网络研讨会上,您将了解到影响显微镜可重复性的因素,有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
AI-based workflow for fast rare event detection in living biological samples using Autonomous Microscopy powered by Aivia

人工智能显微成像能够高效检测稀有事件

对稀有事件进行定位和选择性成像是许多生物样本研究过程的关键。然而,由于时间限制和高度的复杂性,有些实验无法做到,从而限制了获得新发现的前景。通过基于人工智能的显微成像检测稀有事件,这种工作流程将智能样本导航、图像采集工具和人工智能驱动的图像分析等不同功能融合起来共同协作,能够克服上述局限性。
[Translate to chinese:] How is microscopy used in spatial biology - Teaserimage

显微镜如何应用在空间生物学中?一份显微镜指南

本电子书旨在探索显微镜中的关键空间生物学方法,例如多重成像技术,这个方法有助于将独立的细胞信息放入空间环境来分析。
[Translate to chinese:] Five-color FLIM-STED

采用单损耗激光的五色FLIM-STED显微镜

网络研讨会,内容涉及使用单一损耗激光和荧光寿命phasor分离技术的五色STED技术。

Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform

In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…

免疫细胞在组织样品中的共聚焦成像

在本次网络研讨会中,您将探索如何使用共聚焦显微镜对组织样品进行10色成像,并了解这一技术如何有助于评估皮肤免疫状况。
[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing

使用有机荧光团的活细胞荧光寿命多标技术

点播网络研讨会: 如何利用荧光寿命多标技术结合光谱分辨检测技术对更多亚细胞目标进行成像。
Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

荧光寿命成像与荧光共振能量转移

荧光寿命是荧光团在发射荧光光子返回基态之前保持其激发态的平均时间长度。这取决于荧光团的分子组成和纳米环境。 FLIM将寿命测量与成像相结合:对每个图像像素以测得的荧光寿命进行颜色编码,产生额外的图像反差。因此,FLIM可以提供关于荧光分子空间分布的信息和有关其生化状态或纳米环境的信息。…

对囊泡运输的见解

STELLARIS 可为囊泡运输的动态、结构和机理研究提供完整的互补信息。

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
[Translate to chinese:] Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
[Translate to chinese:]

TauInteraction——TauSense新成员,研究分子间相互作用

荧光显微镜是生命科学的重要研究工具之一,用于观察细胞结构和功能。荧光显微镜的一个关键优势在于能够识别多个目标,并能够观察他们之间的相互作用。
Depth coding of endothelial cells in zebrafish eye. Courtesy of Basile Gurchenkov, Imaging Center of the IGBMC, Illkirch-Graffenstaden, France.

Gentle and Flexible 3D Imaging of Biological and Cleared Samples

For 3D imaging of biological samples, light sheet microscopy is a powerful and sensitive tool allowing you to measure (sub)cellular dynamics over long periods of time. Meanwhile, confocal microscopy,…
Identification of distinct structures_roundworm_Ascaris_female

Find Relevant Specimen Details from Overviews

Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
[Translate to chinese:] AiviaMotion: Truly simultaneous multicolor imaging of live cells (U2OS) in 3D

人工智能和共焦显微镜 - 需知信息

本常见问题清单是对AiviaMotion介绍文章“人工智能如何增强共焦成像”的补充,并为相关问题提供了实用的解答。
Dynamic Signal Enhancement powered by Aivia: Truly simultaneous multicolor imaging of live cells (U2OS) in 3D

How Artificial Intelligence Enhances Confocal Imaging

In this article, we show how artificial intelligence (AI) can enhance your imaging experiments. Namely, how Dynamic Signal Enhancement powered by Aivia improves image quality while capturing the…

Spectroscopic Evaluation of Red Blood Cells

Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…

Visualizing Protein Degradation and Aggregation in the Living Cell

Our guest speaker, Prof Dr Eric Reits, presents his work on neurodegenerative disorders. Reits’ group are experts on the subject of Huntington’s disease and work towards identifying leads for…

Life Beyond the Pixels: Deep Learning Methods for Single Cell Analysis

Our guest speaker Prof Dr Peter Horvath presents his work on single cell-based large-scale microscopy experiments. This novel targeting approach includes the use of machine learning models and…
[Translate to chinese:] Nematostella

活细胞成像图库

活细胞显微镜技术是更好地了解细胞和分子功能的基础。如今,宽场显微镜是用于长时间观察细胞动态和发育的最常用技术。共聚焦显微镜也是一种重要工具,可生成三维结构图像,并以高空间和时间分辨率研究高度动态的细胞过程,同时使标本保持接近原生状态。

超分辨率显微镜图片库

由于光的衍射极限,传统共聚焦显微镜无法分辨约240纳米以下的结构。当需要提高分辨率以研究衍射极限尺度以下的结构和分子事件时,会使用超分辨率显微镜技术,如STED、PALM或STORM,或某些解卷积处理方法。

组织图片库

对动物和人体组织进行视觉分析对于了解癌症或神经变性等复杂疾病至关重要。从基本的免疫组化到体内成像,共聚焦显微镜和先进的模式可以让人们了解细胞、生物分子及其在环境中的相互作用。
[Translate to chinese:] Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

神经科学图像

神经科学通常使用显微镜来研究神经系统的功能和了解神经退行性疾病。

多彩图库

荧光多色显微技术是多重成像技术的一个方面,可在同一实验中观察和分析同一样本中的多种元素--每种元素都标记有不同的荧光染料。这不仅能提高实验效率,还能获得更可靠、更有意义的结果,从而了解细胞和组织内的复杂过程。本图集展示了使用THUNDER和STELLARIS平台获得的标有多种荧光探针的样本图像。

癌症研究图片库

荧光显微镜技术使我们能够研究组织和细胞在癌症发展和进展过程中发生的变化。活细胞成像等技术对于了解癌症进展和转移至关重要。

细胞生物学图片库

细胞生物学研究细胞的结构、功能和行为,包括细胞新陈代谢、细胞周期和细胞信号传导。荧光显微镜是细胞生物学家工具包中不可或缺的一部分。宽场显微镜和共聚焦显微镜被广泛用于观察细胞内复杂结构的细节。

Adding Dimensions to Multiplex Molecular Imaging

Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
Root-hypocotyl junction of Arabidopsis thaliana. Image acquired with TauContrast. Sample courtesy: Dr. Melanie Krebs, COS, University of Heidelberg.

Benefits of TauContrast to Image Complex Samples

In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or…

A New World of Confocal Applications with the Next Generation White Light Lasers

As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…
Influenca in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture Influenca virus – red, cilia – green, Nuclei – blue.

How Can Immunofluorescence Aid Virology Research?

Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…

使用 LIGHTNING 可从样本中获得丰富的信息

LIGHTNING 是一个自适应的信息提取过程,可以完全自动化地呈现原本不可见的微小结构和细节。 与为整个图像使用全局参数集的传统技术不同,LIGHTNING 为每一个像素计算一个适当的参数集,尽力还原细节。
Multicolor 3D imaging of live mammalian cell.

Expanding the Frontiers of Confocal Live Cell Imaging

Here we explore how STELLARIS unlocks the full power and potential of live cell studies by overcoming many common limitations and fully integrating fluorescence lifetime-based information to add a new…

Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra

In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample.

STELLARIS白激光

在为多色实验选择荧光探针时,您无需做出妥协。 现在,您可以超越传统激发源,摆脱在荧光团选择和多元成像能力方面的限制。STELLARIS新一代白激光(WLL)与我们提供的Power HyD系列检测器相结合,使您可自由选择所有光谱,并准确组合适当的探针来解答您的实验问题。 白激光与我们自有的声光分束器(AOBS)相结合,可最多同时使用8条独立的扫描激光线。…

TauSense技术成像工具

徕卡显微系统的TauSense技术是一套基于荧光寿命的成像模式。以STELLARIS共焦平台为核心,将彻底改变您的成像实验。无论您的样品或染色程序如何,荧光寿命信息始终存在。现在,TauSense为您提供了获取这些额外信息的途径,并利用不同TauSense模式扩大了您研究潜力的可能性。

Power HyD探测器系列

我们的STELLARIS扩展了探测器技术的极限,使您能够扩展科学研究受到的限制。 我们新设计的Power HyD探测器系列由3种不同的探测器组成,可配置符合您应用需求的共聚焦。

How to Uncover Hidden Dimensions in Research with Lifetime Imaging

Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based…
Fluorescence microscopy image on the left with no distinction between the fluorescent signal and background autofluorescence. FLIM was used in the image on the right to differentiate autofluorescence in chloroplasts (blue) from the desired fluorescent signal from the cell membrane (green).

学习如何从共聚焦图像中去除自发荧光

了解自发荧光的常见原因以及如何将其从共聚焦显微镜图像中去除。根据应用的不同,自发荧光的来源可能有很多种,但幸运的是,同样也有很多的解决方案--从更换介质到使用荧光寿命成像和近红外染料。

DIVE Multiphoton Microscope Image Gallery

Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…

Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic

On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…

Handbook of Optical Filters for Fluorescence Microscopy

Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…

荧光入门介绍

荧光是George Gabriel Stokes于1852年首次报道的一种现象。他观察到萤石在紫外线照射后开始发光。荧光是光致发光的一种形式,是指一种材料被光照射后会发射出光子。发射光的波长比激发光更长。这种效应又称为斯托克斯位移。

应用的领域

活细胞成像

将视角从单一的显微镜组件转向完整的活细胞成像解决方案,徕卡公司将显微镜、LAS X 成像软件、相机和第三方专用组件集成在一起,形成一个完整的活细胞成像系统。

超高分辨率显微镜 STED&GSDIM

徕卡显微系统所提供的超高分辨率显微镜,通过宽场(GSD)和共聚焦(STED)技术克服了衍射极限,是您能够进一步研究亚细胞结构和动态,而这一层级的观察在之前采用普通荧光显微镜是无法实现的。

癌症研究

癌症是一种复杂的异质性疾病,由于细胞生长失控而引起。 一个或一组细胞的基因和表观遗传的变化破坏了正常功能,导致细胞自发、不受控制地生长和增殖。

类器官和3D细胞培养

生命科学研究中最令人振奋的最新进展之一是3D细胞培养系统的发展,例如类器官、球状体或器官芯片模型。 3D细胞培养物是一种人工环境,在这种环境中,细胞能够在三维空间中生长并与周围环境相互作用。 这些环境条件与它们在体内的情况相似。

神经科学研究解决方案

您的工作是更好地了解神经退行性疾病,还是研究神经系统的功能? 了解如何使用徕卡显微系统的成像解决方案取得突破。

荧光

荧光是生物和分析显微镜中最常用的物理现象之一,主要是因为它具有灵敏度高、特异性强的特点。荧光是冷发光的一种形式。用户可以通过显微镜来捕捉单个荧光分子的种类、分布、数量及其在细胞内的定位。用户可以进行荧光分子共定位和相互作用的研究,也可以观察在细胞内和细胞间运作离子浓度的变化,如胞吞和胞吐。借助超高分辨显微镜,我们甚至可以对亚细胞器的结构进行成像。

荧光寿命成像

荧光寿命成像显微术 (FLIM) 是一种利用荧光染料固有特性的成像技术。 除了具有特有的发射光谱外,每个荧光分子还有特有的寿命,它反映了荧光基团在发射光子之前处于激发态的时间。 除了标准的荧光强度测量外,寿命分析还可以提供其他信息。

细胞生物学

如果您的研究重点是探究人类健康和疾病相关的细胞学基础,那么从时空和分子层面详细研究感兴趣的细胞至关重要。 因此,显微成像是细胞生物学中一个非常重要的工具,它让您能够在样本的结构环境中详细研究样本,也可以分析细胞器和大分子。 细胞生物学成像是运用一系列的光学显微镜和电子显微镜完成的。 徕卡显微系统公司推出的成像解决方案专为扩展您的细胞生物学研究而设计。

病毒学

您的主要研究对象是病毒感染和疾病吗? 了解如何使用徕卡显微系统公司的成像和样本制备解决方案深入研究病毒学。

模式生物研究

模式生物是研究人员用来研究特定生物学过程的物种。 它们具有与人类相似的遗传特征,通常用于遗传学、发育生物学和神经科学等研究领域。 选择模式生物的原因通常是它们在实验室环境中易于保持和繁殖、生成周期短,或能够产生突变体来研究某些性状或疾病。
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